Ultra-fast proteomics with Scanning SWATH
Artikel i vetenskaplig tidskrift, 2021

Accurate quantification of the proteome remains challenging for large sample series and longitudinal experiments. We report a data-independent acquisition method, Scanning SWATH, that accelerates mass spectrometric (MS) duty cycles, yielding quantitative proteomes in combination with short gradients and high-flow (800 µl min ) chromatography. Exploiting a continuous movement of the precursor isolation window to assign precursor masses to tandem mass spectrometry (MS/MS) fragment traces, Scanning SWATH increases precursor identifications by ~70% compared to conventional data-independent acquisition (DIA) methods on 0.5–5-min chromatographic gradients. We demonstrate the application of ultra-fast proteomics in drug mode-of-action screening and plasma proteomics. Scanning SWATH proteomes capture the mode of action of fungistatic azoles and statins. Moreover, we confirm 43 and identify 11 new plasma proteome biomarkers of COVID-19 severity, advancing patient classification and biomarker discovery. Thus, our results demonstrate a substantial acceleration and increased depth in fast proteomic experiments that facilitate proteomic drug screens and clinical studies. –1

Författare

Christoph B. Messner

Charité Universitätsmedizin Berlin

The Francis Crick Institute

Vadim Demichev

The Francis Crick Institute

University of Cambridge

Charité Universitätsmedizin Berlin

Nic Bloomfield

MDS SCIEX

Jason S.L. Yu

The Francis Crick Institute

Matthew White

The Francis Crick Institute

Marco Kreidl

The Francis Crick Institute

Anna Sophia Egger

The Francis Crick Institute

Anja Freiwald

Charité Universitätsmedizin Berlin

Gordana Ivosev

MDS SCIEX

Fras Wasim

MDS SCIEX

Aleksej Zelezniak

Chalmers, Biologi och bioteknik, Systembiologi

The Francis Crick Institute

Linda Jürgens

Charité Universitätsmedizin Berlin

Norbert Suttorp

Charité Universitätsmedizin Berlin

Leif Erik Sander

Charité Universitätsmedizin Berlin

Florian Kurth

Universitatsklinikum Hamburg-Eppendorf und Medizinische Fakultat

Charité Universitätsmedizin Berlin

Kathryn S. Lilley

University of Cambridge

Michael Mülleder

Charité Universitätsmedizin Berlin

Stephen Tate

MDS SCIEX

M. Ralser

Charité Universitätsmedizin Berlin

The Francis Crick Institute

Nature Biotechnology

1087-0156 (ISSN)

Vol. In Press

Ämneskategorier

Farmaceutisk vetenskap

Analytisk kemi

Medicinsk bildbehandling

DOI

10.1038/s41587-021-00860-4

PubMed

33767396

Mer information

Senast uppdaterat

2021-05-03