Long-read-sequenced reference genomes of the seven major lineages of enterotoxigenic Escherichia coli (ETEC) circulating in modern time
Artikel i vetenskaplig tidskrift, 2021

Enterotoxigenic Escherichia coli (ETEC) is an enteric pathogen responsible for the majority of diarrheal cases worldwide. ETEC infections are estimated to cause 80,000 deaths annually, with the highest rates of burden, ca 75 million cases per year, amongst children under 5 years of age in resource-poor countries. It is also the leading cause of diarrhoea in travellers. Previous large-scale sequencing studies have found seven major ETEC lineages currently in circulation worldwide. We used PacBio long-read sequencing combined with Illumina sequencing to create high-quality complete reference genomes for each of the major lineages with manually curated chromosomes and plasmids. We confirm that the major ETEC lineages all harbour conserved plasmids that have been associated with their respective background genomes for decades, suggesting that the plasmids and chromosomes of ETEC are both crucial for ETEC virulence and success as pathogens. The in-depth analysis of gene content, synteny and correct annotations of plasmids will elucidate other plasmids with and without virulence factors in related bacterial species. These reference genomes allow for fast and accurate comparison between different ETEC strains, and these data will form the foundation of ETEC genomics research for years to come.

Författare

Astrid von Mentzer

Göteborgs universitet

Wellcome Trust Sanger Institute

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Grace A. Blackwell

European Bioinformatics Institute

Wellcome Trust Sanger Institute

Derek Pickard

University of Cambridge

Christine J. Boinett

Wellcome Trust Sanger Institute

Enrique Joffré

Karolinska Institutet

Andrew J. Page

Institute of Food Research

Wellcome Trust Sanger Institute

Ann Mari Svennerholm

Göteborgs universitet

Gordon Dougan

University of Cambridge

Åsa Sjöling

Karolinska Institutet

Scientific Reports

2045-2322 (ISSN)

Vol. 11 1 9256

Antibiotikaresistens hos diarrébakterier - spridning mellan människa, djur och miljö

Vetenskapsrådet (VR), 2019-01-01 -- 2021-12-31.

Ämneskategorier

Infektionsmedicin

Mikrobiologi inom det medicinska området

Genetik

DOI

10.1038/s41598-021-88316-2

PubMed

33927221

Mer information

Senast uppdaterat

2021-05-20