Herpes simplex virus type 2 mucin-like glycoprotein mgg promotes virus release from the surface of infected cells
Artikel i vetenskaplig tidskrift, 2021

The contribution of virus components to liberation of herpes simplex virus type 2 (HSV-2) progeny virions from the surface of infected cells is poorly understood. We report that the HSV-2 mutant deficient in the expression of a mucin-like membrane-associated glycoprotein G (mgG) exhibited defect in the release of progeny virions from infected cells manifested by ~2 orders of magnitude decreased amount of infectious virus in a culture medium as compared to native HSV-2. Electron microscopy revealed that the mgG deficient virions were produced in infected cells and present at the cell surface. These virions could be forcibly liberated to a nearly native HSV-2 level by the treatment of cells with glycosaminoglycan (GAG)-mimicking oligosaccharides. Comparative assessment of the interaction of mutant and native virions with surface-immobilized chondroitin sulfate GAG chains revealed that while the mutant virions associated with GAGs ~fourfold more extensively, the lateral mobility of bound virions was much poorer than that of native virions. These data indicate that the mgG of HSV-2 balances the virus interaction with GAG chains, a feature critical to prevent trapping of the progeny virions at the surface of infected cells.

glycosaminoglycans

single particle analysis

herpes simplex virus type 2

mucin-like protein

glycoprotein mgG

virus release

Författare

Edward Trybala

Göteborgs universitet

Nadia Peerboom

Chalmers, Fysik, Biologisk fysik

Beata Adamiak

Göteborgs universitet

Malgorzata Krzyzowska

Göteborgs universitet

Jan Åke Liljeqvist

Göteborgs universitet

Marta Bally

Umeå universitet

Tomas Bergström

Göteborgs universitet

Viruses

1999-4915 (ISSN)

Vol. 13 5 887

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Immunologi inom det medicinska området

DOI

10.3390/v13050887

Mer information

Senast uppdaterat

2021-06-10