Dynamic Range Expansion of the C-Reactive Protein Quantification with a Tandem Giant Magnetoresistance Biosensor
Artikel i vetenskaplig tidskrift, 2021

In this study, we report a convenient analytical method for a full-range quantification of the C-reactive protein (CRP), a blood biomarker of infection and cardiovascular events. We determine CRP over the entire diagnostically relevant concentration range in undiluted human blood serum in a single test, using a tandem giant magnetoresistance (GMR) sensor. The tandem principle combines a sandwich assay and a competitive assay, which allows for the discrimination of the concentration values resulting from the multivalued dose-response curve ("Hook"effect), which characterizes the one-step sandwich assay at high CRP concentrations. The sensor covers a linear detection range for CRP concentration from 3 ng/mL to 350 μg/mL, the detection limit (s/n = 3) is 1 ng/mL. The prominent features of the chip-based method are its expanded dynamic range and low sample volume (50 μL), and the need for a short measurement time of 15 min. These figures of merit, in addition to the low detection limit equal to the established assay instrumentation, make it a viable candidate for use in point-of-care diagnostics.


Fanda Meng

Shandong Qianfoshan Hospital

Shandong First Medical University

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Lei Zhang

Chinese Academy of Sciences

Dongguan Bosh Biotechnologies

Weisong Huo

Dongguan Bosh Biotechnologies

Jie Lian

People's Public Security University of China

Aldo Jesorka

Chalmers, Kemi och kemiteknik

Xizeng Shi

Dongguan Bosh Biotechnologies

Yunhua Gao

Chinese Academy of Sciences

ACS Omega

24701343 (eISSN)

Vol. 6 19 12923-12930


Analytisk kemi

Klinisk laboratoriemedicin

Biomedicinsk laboratorievetenskap/teknologi



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