Continuous capillary-flow sensing of glucose and lactate in sweat with an electrochemical sensor based on functionalized graphene oxide
Artikel i vetenskaplig tidskrift, 2021

We describe an electrochemical device for the simultaneous monitoring of glucose and lactate in sweat, based on enzymatic sensors exploiting capillary flow to induce continuous, stable sensing. The enzymes, namely glucose oxidase and lactate oxidase, were anchored to a graphene oxide and chitosan composite (GO-Ch) of original synthesis, to achieve stable deposition of the bioreceptors on the electrochemical platform. We tested both biosensors on a realistic device architecture: they were embedded in a nitrocellulose strip, to exploit capillary force to induce a continuous flux of sweat on the sensor platform, ensuring the constant renewal of sample. We could achieve good sensitivity at potentials close to zero by using Prussian Blue as redox mediator, thus avoiding interference from other chemical species present in the complex matrix. The sensing signal was stable and linear over two hours in a concentration range of glucose and lactate between the limit of quantification (32 and 68 nM, respectively) and the upper limit of linearity (3.8 and 50.0 mM, respectively). The device is simple, robust, stable, and can be easily worn without the direct contact of the active part with the skin, making it suitable for simultaneous monitoring of glucose and lactate in human sweat.

Capillary flux

Functionalized graphene oxide

Dual electrode

Enzymatic detection

Wearable biosensors

Biomarkers detection

Författare

Fabrizio Poletti

Universita Degli Studi Di Modena E Reggio Emilia

Barbara Zanfrognini

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

Laura Favaretto

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

Vanesa Quintano

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

Jinhua Sun

Chalmers, Industri- och materialvetenskap, Material och tillverkning

E. Treossi

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

M. Melucci

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

Vincenzo Palermo

Chalmers, Industri- och materialvetenskap, Material och tillverkning

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

Chiara Zanardi

Universita Degli Studi Di Modena E Reggio Emilia

Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR)

Sensors and Actuators, B: Chemical

0925-4005 (ISSN)

Vol. 344 130253

Ämneskategorier

Analytisk kemi

Textil-, gummi- och polymermaterial

Biokatalys och enzymteknik

DOI

10.1016/j.snb.2021.130253

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Senast uppdaterat

2021-06-24