Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)
Artikel i vetenskaplig tidskrift, 2021

Synthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C12 chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional ∆9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T2 generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T4 generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone.


Agrobacterium-based floral-dip transformation

Plant factory

Acyl-ACP thioesterase

Multi-gene copies


∆9 desaturase

Conjugated double bonds


Yihan Xia

Chalmers, Biologi och bioteknik, Systembiologi

Lunds universitet

Hong Lei Wang

Lunds universitet

Bao Jian Ding

Lunds universitet

Glenn P. Svensson

Lunds universitet

Carin Jarl-Sunesson

Lunds universitet

Edgar B. Cahoon

University of Nebraska - Lincoln

Per Hofvander

Sveriges lantbruksuniversitet (SLU)

Christer Löfstedt

Lunds universitet

Journal of Chemical Ecology

0098-0331 (ISSN) 1573-1561 (eISSN)

Vol. 47 12 950-967




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