Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)
Artikel i vetenskaplig tidskrift, 2021

Synthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C12 chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional ∆9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T2 generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T4 generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone.

Bioassay

Agrobacterium-based floral-dip transformation

Plant factory

Acyl-ACP thioesterase

Multi-gene copies

P19

∆9 desaturase

Conjugated double bonds

Författare

Yihan Xia

Chalmers, Biologi och bioteknik, Systembiologi

Lunds universitet

Hong Lei Wang

Lunds universitet

Bao Jian Ding

Lunds universitet

Glenn P. Svensson

Lunds universitet

Carin Jarl-Sunesson

Lunds universitet

Edgar B. Cahoon

University of Nebraska - Lincoln

Per Hofvander

Sveriges lantbruksuniversitet (SLU)

Christer Löfstedt

Lunds universitet

Journal of Chemical Ecology

0098-0331 (ISSN) 1573-1561 (eISSN)

Vol. In Press

Ämneskategorier

Växtbioteknologi

Zoologi

Organisk kemi

DOI

10.1007/s10886-021-01316-4

PubMed

34762210

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Senast uppdaterat

2021-11-23