Graphene-based sensor for detection of bacterial pathogens
Artikel i vetenskaplig tidskrift, 2021

Microbial colonization to biomedical surfaces and biofilm formation is one of the key challenges in the medical field. Recalcitrant biofilms on such surfaces cause serious infections which are difficult to treat using antimicrobial agents, due to their complex structure. Early detection of microbial colonization and monitoring of biofilm growth could turn the tide by providing timely guidance for treatment or replacement of biomedical devices. Hence, there is a need for sensors, which could generate rapid signals upon bacterial colonization. In this study, we developed a simple prototype sensor based on pristine, non-functionalized graphene. The detection principle is a change in electrical resistance of graphene upon exposure to bacterial cells. Without functionalization with specific receptors, such sensors cannot be expected to be selective to certain bacteria. However, we demonstrated that two different bacterial species can be detected and differentiated by our sensor due to their different growth dynamics, adherence pattern, density of adhered bacteria and microcolonies formation. These distinct behaviors of tested bacteria depicted distinguishable pattern of resistance change, resistance versus gate voltage plot and hysteresis effect. This sensor is simple to fabricate, can easily be miniaturized, and can be effective in cases when precise identification of species is not needed.

Staphylococcus epidermidis

Graphene

Sensors

Biofilms

Pseudomonas aeruginosa

Författare

Santosh Pandit

Chalmers, Biologi och bioteknik, Systembiologi

Mengyue Li

Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik

Yanyan Chen

Chalmers, Biologi och bioteknik, Systembiologi

Shadi Rahimi

Chalmers, Biologi och bioteknik, Systembiologi

Venkata Raghavendra Subrahmanya Sar Mokkapati

Chalmers, Biologi och bioteknik, Systembiologi

Alessandra Merlo

Chalmers, Biologi och bioteknik, Systembiologi

Avgust Yurgens

Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik

Ivan Mijakovic

Chalmers, Biologi och bioteknik, Systembiologi

Danmarks Tekniske Universitet (DTU)

Sensors

1424-8220 (ISSN) 1424-3210 (eISSN)

Vol. 21 23 8085

Ämneskategorier

Infektionsmedicin

Mikrobiologi

Mikrobiologi inom det medicinska området

DOI

10.3390/s21238085

PubMed

34884089

Mer information

Senast uppdaterat

2022-01-14