Rapid in-plate screening of biotransformation products in single zebrafish embryos
Artikel i vetenskaplig tidskrift, 2021

A procedure was developed for rapid screening of xenobiotic biotransformation products (bioTPs) in single zebrafish (ZF; Danio rerio) embryos. Exposure was carried out from 0–120 hours post fertilization (hpf) to 6 different concentrations of the model compound propranolol (PPL). Following in-plate extraction and non-target instrumental analysis by high resolution mass spectrometry, suspected bioTPs were identified using custom data filtration scripts and matching to in silico structural predictions. A total of eight PPL bioTPs were identified (five at a level 1 confidence and one at a level 2–3 confidence). These findings supplement previously generated toxicometabolomic models derived from the same dataset, and were obtained without conducting additional exposure experiments. In addition to facilitating assessments of inter-individual variability in bioTP production in ZF embryos, we demonstrate that bioTPs can be elucidated using extremely small quantities of biomass (i.e. ∼200 μg). To the best of our knowledge, this is the first time bioTP elucidation has been carried out in single ZF embryos.



Non-targeted analysis


Zebrafish embryo


Anton Ribbenstedt

Stockholms universitet

Jonathan P. Benskin

Stockholms universitet

RSC Advances

2046-2069 (ISSN)

Vol. 11 45 27812-27819


Biokemi och molekylärbiologi

Analytisk kemi

Annan kemi

Bioinformatik (beräkningsbiologi)


Grundläggande vetenskaper


Livsvetenskaper och teknik (2010-2018)



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