Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
Artikel i vetenskaplig tidskrift, 2022

Background:
Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management.
Results:
In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant.
Conclusions:
Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

Fatty acyl reductase

Acetyltransferase

Heterologous expression systems

Functional characterization

Alcohol oxidation

Pheromone-releasing plants

Fatty acyl desaturases

Författare

Yihan Xia

Chalmers, Biologi och bioteknik, Systembiologi

Lunds universitet

Bao Jian Ding

Lunds universitet

Shuang Lin Dong

Nanjing Agricultural University

Hong Lei Wang

Lunds universitet

Per Hofvander

Sveriges lantbruksuniversitet (SLU)

Christer Löfstedt

Lunds universitet

BMC Biology

1741-7007 (ISSN)

Vol. 20 1 80

Ämneskategorier

Växtbioteknologi

Zoologi

Genetik

DOI

10.1186/s12915-022-01281-8

PubMed

35361182

Mer information

Senast uppdaterat

2022-04-14