Multi-omics analyses of the transition to the Crabtree effect in S. cerevisiae reveals a key role for the citric acid shuttle
Artikel i vetenskaplig tidskrift, 2022

The article investigates the Crabtree effect under dynamic process which have never been reported, and find the citric acid shuttles to support higher demand of alpha-ketoglutarate under Crabtree effect. The Crabtree effect in the yeast, Saccharomyces cerevisiae, has been extensively studied, but only few studies have analyzed the dynamic conditions across the critical specific growth rate where the Crabtree effect sets in. Here, we carried out a multi-omics analysis of S. cerevisiae undergoing a specific growth rate transition from 0.2 h(-1) to 0.35 h(-1). The extracellular metabolome, the transcriptome and the proteome were analyzed in an 8-h transition period after the specific growth rate shifted from 0.2 h(-1) to 0.35 h(-1). The changing trends of both the transcriptome and proteome were analyzed using principal component analysis, which showed that the transcriptome clustered together after 60 min, while the proteome reached steady-state much later. Focusing on central carbon metabolism, we analyzed both the changes in the transcriptome and proteome, and observed an interesting changing pattern in the tricarboxylic acid (TCA) pathway, which indicates an important role for citric acid shuttling across the mitochondrial membrane for alpha-ketoglutarate accumulation during the transition from respiratory to respiro-fermentative metabolism. This was supported by a change in the oxaloacetate and malate shuttle. Together, our findings shed new light into the onset of the Crabtree effect in S. cerevisiae.

Saccharomyces cerevisiae

TCA cycle

Crabtree effect

Multi-omics

Författare

Tingting Xie

East China University of Science and Technology

Min Chen

East China University of Science and Technology

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

BioInnovation Institute

Jianye Xia

East China University of Science and Technology

Chinese Academy of Sciences

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 22 1 foac030

Ämneskategorier

Industriell bioteknik

DOI

10.1093/femsyr/foac030

PubMed

35595470

Mer information

Senast uppdaterat

2022-07-18