Screening of xylose utilizing and high lipid producing yeast strains as a potential candidate for industrial application
Artikel i vetenskaplig tidskrift, 2022
RESULTS: By collecting leaves from exotic plants in greenhouses and selective cultivation on xylose, a high frequency of oleaginous yeasts was obtained (> 40%). Different cultivation conditions lead to differences in fatty acid contents and compositions, resulting in a set of strains that can be used to select candidate production strains for different purposes. In this study, the most prominent strains were identified as Pseudozyma hubeiensis BOT-O and Rhodosporidium toruloides BOT-A2. The fatty acid levels per cell dry weight after cultivation in a nitrogen limited medium with either glucose, xylose or glycerol as carbon source, respectively, were 46.8, 43.2 and 38.9% for P. hubeiensis BOT-O, and 40.4, 27.3 and 42.1% for BOT-A2. Furthermore, BOT-A2 accumulated 45.1% fatty acids per cell dry weight in a natural plant hydrolysate, and P. hubeiensis BOT-O showed simultaneous glucose and xylose consumption with similar growth rates on both carbon sources. The fatty acid analysis demonstrated both long chain and poly-unsaturated fatty acids, depending on strain and medium.
CONCLUSIONS: We found various natural yeast isolates with high lipid production capabilities and the ability to grow not only on glucose, but also xylose, glycerol and natural plant hydrolysate. R. toruloides BOT-A2 and P. hubeiensis BOT-O specifically showed great potential as production strains with high levels of storage lipids and comparable growth to that on glucose on various other substrates, especially compared to currently used lipid production strains. In BOT-O, glucose repression was not detected, making it particularly desirable for utilization of plant waste hydrolysates. Furthermore, the isolated strains were shown to produce oils with fatty acid profiles similar to that of various plant oils, making them interesting for future applications in fuel, food or feed production.
Pseudozyma hubeiensis
Lignocellulose
Oleaginous yeast
Microbial lipids
Rhodosporidium toruloides
Författare
Linnea Qvirist
Chalmers, Biologi och bioteknik, Livsmedelsvetenskap
Friederike Mierke
Chalmers, Biologi och bioteknik, Livsmedelsvetenskap
Ricardo Vázques-Juárez
Centro de Investigaciones Biologicas Del Noroeste
Thomas Andlid
Chalmers, Biologi och bioteknik, Livsmedelsvetenskap
BMC Microbiology
14712180 (eISSN)
Vol. 22 1 173-Ämneskategorier
Kemiska processer
Bioenergi
Mikrobiologi
DOI
10.1186/s12866-022-02586-y
PubMed
35799117