DOM Molecular Weight Fractionation and Fluorescence Quantum Yield Assessment Using a Coupled In-Line SEC Optical Property System
Artikel i vetenskaplig tidskrift, 2022

Size exclusion chromatography (SEC) in combination with optical measurements has become a popular form of analysis to characterize dissolved organic matter (DOM) as a function of molecular size. Here, SEC coupled with in-line absorbance scans and fluorescence emission scans was utilized to derive apparent fluorescence quantum yield (φf) as a function of molecular weight (MW) for DOM. Individual instrument-specific SEC-fluorescence detector correction factors were developed by comparison of an SEC-based excitation emission matrix (EEM) to an EEM generated by a calibrated benchtop fluorometer. The method was then applied to several sample sets to demonstrate how to measure the φf of unknown DOM samples and to observe changes to φf following a processing mechanism (ozonation). The φf of riverine water samples and DOM fulvic acid isolates from Suwannee River and Pony Lake increased from < 0.5% to a maximum of 2.5-3% across the medium- to low-MW range. Following ozonation of PLFA, φf increased most notably in the large-MW fractions (elution volumes < 40 mL). Overall, this method provides a means by which highly fluorescent size fractions of DOM can be identified for more detailed analyses of chemical composition and its changes through different processing mechanisms.

fluorescence

ozone

size exclusion chromatography

quantum yield

dissolved organic matter

optical properties

Författare

Blair Hanson

University of Colorado at Boulder

Urban Wuensch

Chalmers, Arkitektur och samhällsbyggnadsteknik, Vatten Miljö Teknik

Danmarks Tekniske Universitet (DTU)

Shelby Buckley

University of Colorado at Boulder

Sarah Fischer

University of Missouri

University of Colorado at Boulder

Frank Leresche

University of Colorado at Boulder

Kathleen Murphy

Chalmers, Arkitektur och samhällsbyggnadsteknik, Vatten Miljö Teknik

Juliana D'Andrilli

Louisiana Universities Marine Consortium

Fernando L. Rosario-Ortiz

University of Colorado at Boulder

ACS ES and T Water

26900637 (eISSN)

Vol. 2 12 2491-2501

Förbättrad specificitet för övervakning av dricksvattenbehandling

Formas (2017-00743), 2018-01-01 -- 2020-12-31.

Ämneskategorier

Polymerkemi

Analytisk kemi

Atom- och molekylfysik och optik

DOI

10.1021/acsestwater.2c00318

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Senast uppdaterat

2024-03-07