SAXS imaging reveals optimized osseointegration properties of bioengineered oriented 3D-PLGA/aCaP scaffolds in a critical size bone defect model
Artikel i vetenskaplig tidskrift, 2023
Healing large bone defects remains challenging in orthopedic surgery and is often associated with poor outcomes and complications. A major issue with bioengineered constructs is achieving a continuous interface between host bone and graft to enhance biological processes and mechanical stability. In this study, we have developed a new bioengineering strategy to produce oriented biocompatible 3D PLGA/aCaP nanocomposites with enhanced osseointegration. Decellularized scaffolds -containing only extracellular matrix- or scaffolds seeded with adipose-derived mesenchymal stromal cells were tested in a mouse model for critical size bone defects. In parallel to micro-CT analysis, SAXS tensor tomography and 2D scanning SAXS were employed to determine the 3D arrangement and nanostructure within the critical-sized bone. Both newly developed scaffold types, seeded with cells or decellularized, showed high osseointegration, higher bone quality, increased alignment of collagen fibers and optimal alignment and size of hydroxyapatite minerals.
SAXS tomography
PLGA/aCaP
Extracellular matrix
Critical size bone defect
Scaffold
Mesenchymal stromal cells
Författare
Elisa A. Casanova
Universitätsspital Zürich
Adrian Rodriguez Palomo
Chalmers, Fysik, Materialfysik
Lisa Stähli
Universitätsspital Zürich
Kevin Arnke
Universitätsspital Zürich
Olivier Gröninger
Eidgenössische Technische Hochschule Zürich (ETH)
Melanie Generali
Universität Zürich
Yvonne Neldner
Universitätsspital Zürich
Simon Tiziani
Universitätsspital Zürich
Ana Perez Dominguez
Universität Zürich
Manuel Guizar-Sicairos
Paul Scherrer Institut
Zirui Gao
Paul Scherrer Institut
Christian Appel
Paul Scherrer Institut
Leonard Nielsen
Chalmers, Fysik, Materialfysik
Marios Georgiadis
Stanford University
Franz E. Weber
Universität Zürich
Wendelin Stark
Eidgenössische Technische Hochschule Zürich (ETH)
Hans Christoph Pape
Universitätsspital Zürich
Paolo Cinelli
Universität Zürich
Universitätsspital Zürich
Marianne Liebi
Chalmers, Fysik, Materialfysik
Eidgenössische Materialprüfungs- und Forschungsanstalt (Empa)
Biomaterials
0142-9612 (ISSN) 18785905 (eISSN)
Vol. 294 121989Ämneskategorier
Biomaterial
Biomaterialvetenskap
Medicinska material och protesteknik
DOI
10.1016/j.biomaterials.2022.121989
PubMed
36628888