SAXS imaging reveals optimized osseointegration properties of bioengineered oriented 3D-PLGA/aCaP scaffolds in a critical size bone defect model
Artikel i vetenskaplig tidskrift, 2023

Healing large bone defects remains challenging in orthopedic surgery and is often associated with poor outcomes and complications. A major issue with bioengineered constructs is achieving a continuous interface between host bone and graft to enhance biological processes and mechanical stability. In this study, we have developed a new bioengineering strategy to produce oriented biocompatible 3D PLGA/aCaP nanocomposites with enhanced osseointegration. Decellularized scaffolds -containing only extracellular matrix- or scaffolds seeded with adipose-derived mesenchymal stromal cells were tested in a mouse model for critical size bone defects. In parallel to micro-CT analysis, SAXS tensor tomography and 2D scanning SAXS were employed to determine the 3D arrangement and nanostructure within the critical-sized bone. Both newly developed scaffold types, seeded with cells or decellularized, showed high osseointegration, higher bone quality, increased alignment of collagen fibers and optimal alignment and size of hydroxyapatite minerals.

SAXS tomography

PLGA/aCaP

Extracellular matrix

Critical size bone defect

Scaffold

Mesenchymal stromal cells

Författare

Elisa A. Casanova

Universitätsspital Zürich

Adrian Rodriguez Palomo

Chalmers, Fysik, Materialfysik

Lisa Stähli

Universitätsspital Zürich

Kevin Arnke

Universitätsspital Zürich

Olivier Gröninger

Eidgenössische Technische Hochschule Zürich (ETH)

Melanie Generali

Universität Zürich

Yvonne Neldner

Universitätsspital Zürich

Simon Tiziani

Universitätsspital Zürich

Ana Perez Dominguez

Universität Zürich

Manuel Guizar-Sicairos

Paul Scherrer Institut

Zirui Gao

Paul Scherrer Institut

Christian Appel

Paul Scherrer Institut

Leonard Nielsen

Chalmers, Fysik, Materialfysik

Marios Georgiadis

Stanford University

Franz E. Weber

Universität Zürich

Wendelin Stark

Eidgenössische Technische Hochschule Zürich (ETH)

Hans Christoph Pape

Universitätsspital Zürich

Paolo Cinelli

Universität Zürich

Universitätsspital Zürich

Marianne Liebi

Chalmers, Fysik, Materialfysik

Eidgenössische Materialprüfungs- und Forschungsanstalt (Empa)

Biomaterials

0142-9612 (ISSN) 18785905 (eISSN)

Vol. 294 121989

Ämneskategorier

Biomaterial

Biomaterialvetenskap

Medicinska material och protesteknik

DOI

10.1016/j.biomaterials.2022.121989

PubMed

36628888

Mer information

Senast uppdaterat

2023-01-16