Substrate-derived Sortase A inhibitors: targeting an essential virulence factor of Gram-positive pathogenic bacteria
Artikel i vetenskaplig tidskrift, 2023

The bacterial transpeptidase Sortase A (SrtA) is a surface enzyme of Gram-positive pathogenic bacteria. It has been shown to be an essential virulence factor for the establishment of various bacterial infections, including septic arthritis. However, the development of potent Sortase A inhibitors remains an unmet challenge. Sortase A relies on a five amino acid sorting signal (LPXTG), by which it recognizes its natural target. We report the synthesis of a series of peptidomimetic inhibitors of Sortase A based on the sorting signal, supported by computational binding analysis. By employing a FRET-compatible substrate, our inhibitors were assayed in vitro. Among our panel, we identified several promising inhibitors with IC50 values below 200 mu M, with our strongest inhibitor - LPRDSar - having an IC50 of 18.9 mu M. Furthermore, it was discovered that three of our compounds show an effect on growth and biofilm inhibition of pathogenic Staphylococcus aureus, with the inclusion of a phenyl ring seemingly key to this effect. The most promising compound in our panel, BzLPRDSar, could inhibit biofilm formation at concentrations as low as 32 mu g mL(-1), manifesting it as a potential future drug lead. This could lead to treatments for MRSA infections in clinics and diseases such as septic arthritis, which has been directly linked with SrtA.

Författare

Helal Abujubara

Göteborgs universitet

Jordi C. J. Hintzen

Göteborgs universitet

Shadi Rahimi

Chalmers, Life sciences, Systembiologi

Ivan Mijakovic

Novo Nordisk Fonden

Chalmers, Life sciences, Systembiologi

Daniel Tietze

Göteborgs universitet

Alesia A. Tietze

Göteborgs universitet

Chemical Science

2041-6520 (ISSN) 2041-6539 (eISSN)

Vol. 14 25 6975-6985

Ämneskategorier

Mikrobiologi inom det medicinska området

DOI

10.1039/d3sc01209c

Mer information

Senast uppdaterat

2024-03-07