Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization
Artikel i vetenskaplig tidskrift, 2024

Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled in complex natural sample environments. Here, we introduce dual-angle interferometric scattering microscopy (DAISY), enabling optical quantification of both size and polarizability of individual nanoparticles (radius <170 nm) without requiring a priori information regarding the surrounding media or super-resolution imaging. DAISY achieves this by combining the information contained in concurrently measured forward and backward scattering images through twilight off-axis holography and interferometric scattering (iSCAT). Going beyond particle size and polarizability, single-particle morphology can be deduced from the fact that the hydrodynamic radius relates to the outer particle radius, while the scattering-based size estimate depends on the internal mass distribution of the particles. We demonstrate this by differentiating biomolecular fractal aggregates from spherical particles in fetal bovine serum at the single-particle level.

nanoparticles

holography

aggregates

size

morphology

iSCAT

Författare

Erik Olsén

Chalmers, Fysik, Nano- och biofysik

Berenice García Rodríguez

Göteborgs universitet

Fredrik Skärberg

Göteborgs universitet

Petteri Parkkila

Chalmers, Fysik, Nano- och biofysik

Giovanni Volpe

Göteborgs universitet

Fredrik Höök

Chalmers, Fysik, Nano- och biofysik

Daniel Sundås Midtvedt

Göteborgs universitet

Nano Letters

1530-6984 (ISSN) 1530-6992 (eISSN)

Vol. 24 6 1874-1881

Ämneskategorier

Atom- och molekylfysik och optik

DOI

10.1021/acs.nanolett.3c03539

Mer information

Senast uppdaterat

2024-03-07