Redesign and reconstruction of a mevalonate pathway and its application in terpene production in Escherichia coli
Artikel i vetenskaplig tidskrift, 2019

Metabolic engineering of heterologous biosynthetic pathways in microbial hosts is an alternative way for producing high-value biochemicals. Here, heterologous mevalonate (MVA) and prenyl pyrophosphate (IPP) pathways of a new isolate Enterococcus kingsejongensis Gf1 were redesigned and reconstructed in Escherichia coli. Two MVA upstream enzymes of E. kingsejongensis Gf1 were functionally expressed and overexpression of the two enzymes led to a yield of 52.5 g/L MVA after 65 h of fed-batch fermentation. Next, the functions of three MVA downstream enzymes were verified by addition of exogenous MVA. Finally, whole MVA pathway enzymes and IPP pathway enzymes were combined in E. coli in order to overproduce squalene and β-carotene. The E. coli strains coexpressing entire MVA and IPP pathway enzymes enhanced squalene production by 15-fold and β-carotene production by 2.5-fold.

EnterococcusPathway engineeringMevalonate

Författare

BoHyun Choi

Chalmers, Biologi och bioteknik, Industriell bioteknik

JinHo Kim

Chalmers, Biologi och bioteknik, Systembiologi

Sun Young Choi

Ajou University

Se Jong Han

Korea Polar Research Institute

Pyung Cheon Lee

Ajou University

Bioresource Technology Reports

2589014X (eISSN)

Vol. 7 100291

Ämneskategorier

Bioprocessteknik

Bioinformatik och systembiologi

Organisk kemi

DOI

10.1016/j.biteb.2019.100291

Mer information

Skapat

2024-05-08