Increased dietary protein stimulates amino acid catabolism via the gut microbiota and secondary bile acid production
Artikel i vetenskaplig tidskrift, 2025

Excess amino acids from a protein-rich diet are mainly catabolized in the liver. However, it is still unclear to what extent the gut microbiota may be involved in the mechanisms governing this catabolism. Therefore, the aim of this study was to investigate whether consumption of different dietary protein concentrations induces changes in the taxonomy of the gut microbiota, which may contribute to the regulation of hepatic amino acid catabolism. Consumption of a high-protein diet caused overexpression of HIF-1 alpha in the colon and increase in mitochondrial activity, creating a more anaerobic environment that was associated with changes in the taxonomy of the gut microbiota promoting an increase in the synthesis of secondary bile acids, increased secretion of pancreatic glucagon. This effect was demonstrated in pancreatic islets, where secondary bile acids stimulated the expression of the PC2 enzyme that promotes glucagon formation. The increase in circulating glucagon was associated with an induction of the expression of hepatic amino acid-degrading enzymes, an effect attenuated by antibiotics. Thus, high protein intake in mice and humans induced the increase of different species in the gut microbiota with the capacity to produce secondary bile acids leading to an increase in secondary bile acids and glucagon levels, promoting amino acid catabolism.

amino acid catabolism

Gut microbiota

secondary bile acids

glucagon

high-protein diet

Författare

Sandra Tobon-Cornejo

Instituto Nacional de la Nutricion Salvador Zubiran

Mónica Sánchez-Tapia

Instituto Nacional de la Nutricion Salvador Zubiran

Rocio Guizar-Heredia

Instituto Nacional de la Nutricion Salvador Zubiran

Laura Velazquez Villegas

Instituto Nacional de la Nutricion Salvador Zubiran

Lilia G. Noriega

Instituto Nacional de la Nutricion Salvador Zubiran

Janette Furuzawa-Carballeda

Instituto Nacional de la Nutricion Salvador Zubiran

Rogelio Hernandez-Pando

Instituto Nacional de la Nutricion Salvador Zubiran

Natalia Vazquez-Manjarrez

Instituto Nacional de la Nutricion Salvador Zubiran

Omar Granados-Portillo

Instituto Nacional de la Nutricion Salvador Zubiran

Adriana Lopez-Barradas

Instituto Nacional de la Nutricion Salvador Zubiran

Rosa Rebollar-Vega

Instituto Nacional de la Nutricion Salvador Zubiran

Universidad Nacional Autónoma de México

Otoniel Maya Lucas

Chalmers, Fysik, E-commons

Aaron W. Miller

Cleveland Clinic Foundation

Aurora Serralde

Instituto Nacional de la Nutricion Salvador Zubiran

Martha Guevara-Cruz

Instituto Nacional de la Nutricion Salvador Zubiran

Nimbe Torres

Instituto Nacional de la Nutricion Salvador Zubiran

Armando R. Tovar

Instituto Nacional de la Nutricion Salvador Zubiran

Gut Microbes

1949-0976 (ISSN) 1949-0984 (eISSN)

Vol. 17 1 2465896

Ämneskategorier (SSIF 2025)

Gastroenterologi och hepatologi

Näringslära och dietkunskap

Endokrinologi och diabetes

DOI

10.1080/19490976.2025.2465896

PubMed

39980327

Mer information

Senast uppdaterat

2025-03-14