Spatiotemporal single-cell roadmap of human skin wound healing
Artikel i vetenskaplig tidskrift, 2025

Wound healing is vital for human health, yet the details of cellular dynamics and coordination in human wound repair remain largely unexplored. To address this, we conducted single-cell multi-omics analyses on human skin wound tissues through inflammation, proliferation, and remodeling phases of wound repair from the same individuals, monitoring the cellular and molecular dynamics of human skin wound healing at an unprecedented spatiotemporal resolution. This singular roadmap reveals the cellular architecture of the wound margin and identifies FOSL1 as a critical driver of re-epithelialization. It shows that pro-inflammatory macrophages and fibroblasts sequentially support keratinocyte migration like a relay race across different healing stages. Comparison with single-cell data from venous and diabetic foot ulcers uncovers a link between failed keratinocyte migration and impaired inflammatory response in chronic wounds. Additionally, comparing human and mouse acute wound transcriptomes underscores the indispensable value of this roadmap in bridging basic research with clinical innovations.

scRNA-seq

acute wound

chronic wounds

diabetic foot ulcer

wound healing

venous ulcer

spatial transcriptomics

Författare

Zhuang Liu

Karolinska Institutet

Xiaowei Bian

Karolinska Institutet

Lihua Luo

Karolinska Institutet

Åsa Björklund

Chalmers, Life sciences, Systembiologi

National Bioinformatics Infrastructure Sweden (NBIS)

Li Li

Chinese Academy of Medical Sciences

Letian Zhang

Karolinska Institutet

Yongjian Chen

Karolinska Institutet

Lei Guo

Chinese Academy of Medical Sciences

Juan Gao

Chinese Academy of Medical Sciences

Chunyan Cao

Chinese Academy of Medical Sciences

Jiating Wang

Chinese Academy of Medical Sciences

Wenjun He

Soochow University

Yunting Xiao

Chinese Academy of Medical Sciences

Liping Zhu

Chinese Academy of Medical Sciences

Karl Annusver

Karolinska Institutet

Nusayhah Hudaa Gopee

Newcastle University

Daniela Basurto-Lozada

Wellcome Trust Sanger Institute

Newcastle University

David Horsfall

Newcastle University

Wellcome Trust Sanger Institute

Clare L. Bennett

University College London (UCL)

Maria Kasper

Karolinska Institutet

Muzlifah Haniffa

NHS Foundation Trust

Wellcome Trust Sanger Institute

Newcastle University

Pehr Sommar

Karolinska universitetssjukhuset

Dongqing Li

Chinese Academy of Medical Sciences

Ning Xu Landén

Karolinska Institutet

Cell Stem Cell

1934-5909 (ISSN) 18759777 (eISSN)

Vol. 32 3 479-498.e8

Ämneskategorier (SSIF 2025)

Cell- och molekylärbiologi

Dermatologi och venereologi

Kirurgi

DOI

10.1016/j.stem.2024.11.013

PubMed

39729995

Mer information

Senast uppdaterat

2025-03-21