The molecular landscape of cellular metal ion biology
Artikel i vetenskaplig tidskrift, 2025

Metal ions have crucial roles in cells, but the impact of their availability on biological networks is underexplored. We systematically quantified yeast cell growth and the corresponding metallomic, proteomic, and genetic responses to perturbations in metal availability along concentration gradients of all growth-essential metal ions. We report a remarkable metal concentration dependency of cellular networks, with around half of the proteome, and most signaling pathways such as target of rapamycin (TOR), being metal responsive. Although the biological response to each metal is distinct, our data reveal common properties of metal responsiveness, such as concentration interdependencies and metal homeostasis. Furthermore, our resource indicates that many understudied proteins have functions related to metal biology and reveals that metalloenzymes occupy central nodes in metabolic networks. This work provides a framework for understanding the critical role of metal ions in cellular function, with broader implications for manipulating metal homeostasis in biotechnology and medicine.

metal responsiveness

gene function prediction

metal signaling

metal ion biology

calcium poorly characterized proteins

iron

metabolism

copper

zinc

Författare

Simran Kaur Aulakh

University of Oxford

The Francis Crick Institute

Oliver Lemke

Charité Universitätsmedizin Berlin

Berliner Institut für Gesundheitsforschung

Lukasz Szyrwiel

Charité Universitätsmedizin Berlin

Stephan Kamrad

The Francis Crick Institute

Yu Chen

Shenzhen Institute of Advanced Technology

Chalmers, Life sciences, Systembiologi

Johannes Hartl

Charité Universitätsmedizin Berlin

Berliner Institut für Gesundheitsforschung

Michael Mülleder

Charité Universitätsmedizin Berlin

Jens B Nielsen

BioInnovation Institute

Chalmers, Life sciences, Systembiologi

M. Ralser

Charité Universitätsmedizin Berlin

The Francis Crick Institute

University of Oxford

Berliner Institut für Gesundheitsforschung

Max-Planck-Gesellschaft

Cell Systems

24054712 (ISSN) 24054720 (eISSN)

Vol. In Press 101319

Ämneskategorier (SSIF 2025)

Molekylärbiologi

DOI

10.1016/j.cels.2025.101319

Mer information

Senast uppdaterat

2025-06-25