Utilising a genetically encoded biosensor for in-line monitoring of H2O2 generation during methanol assimilation in the yeast Komagataella phaffii

Övrigt konferensbidrag, 2025

The interest in the methylotrophic yeast Komagataella phaffii (syn Pichia pastoris) has been increasing significantly over the years. Presenting unique features, such as high secretory capabilities and ability to utilise methanol as carbon source, K. phaffii is a very interesting non-conventional yeast to be used in the contexts of recombinant protein production and methanol-based technologies.
Generated either during respiration, oxidative protein folding in the endoplasmic reticulum or during the initial steps of methanol assimilation via the alcohol oxidase enzymes, H₂O₂ can be significantly toxic to the cells, and at high levels cause not only the disruption of different cellular activities, but also damage membranes and organelles. Therefore, a better understanding and monitoring of this non-radical reactive oxygen species (ROS) is essential. Dye-based detection of H₂O₂ is often ambiguous and does not allow for real-time measurements.