The return of metabolism: biochemistry and physiology of glycolysis
Artikel i vetenskaplig tidskrift, 2025
Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and alternative glycolytic routes. By examining glycolysis across the tree of life, we explore its presence and adaptation in prokaryotes, archaea, bacteria, animals and plants, and the extension of glycolysis into sulfosugar metabolism. Further, we discuss the role of unwanted side reactions, thermodynamic principles, and metabolic control principles that underpin glycolysis and the broader metabolic network, and summarise advanced methods for quantifying glycolytic activity, including new analytical methods, alongside kinetic, constraint-based, and machine-learning based modelling. With a focus on the Pasteur, Crabtree, and Warburg effects, this review further discusses the roles of glycolysis in health and disease, highlighting its impact on global metabolic operations, inborn errors, and various pathologies as well as its role in biotechnology and metabolic engineering.
Crabtree effect
metabolic diseases
energy metabolism
Pasteur effect
Warburg effect
thermodynamics
glycolysis
computational modelling
biochemical pathway
metabolic regulation
Författare
Nana-Maria Grüning
Charité Universitätsmedizin Berlin
Federica Agostini
Charité Universitätsmedizin Berlin
Camila Caldana
Max-Planck-Gesellschaft
Johannes Hartl
Charité Universitätsmedizin Berlin
Matthias Heinemann
Rijksuniversiteit Groningen
M. A. Keller
Medizinische Universität Innsbruck
Jan Lukas Krusemann
Charité Universitätsmedizin Berlin
Max-Planck-Gesellschaft
Costanza Lamperti
Carlo Besta Neurological Institute
Carole L. Linster
Université du Luxembourg
Steffen N. Lindner
Charité Universitätsmedizin Berlin
Julia Muenzner
Charité Universitätsmedizin Berlin
Jens B Nielsen
BioInnovation Institute
Chalmers, Life sciences, Systembiologi
Zoran Nikoloski
Max-Planck-Gesellschaft
Universität Potsdam
Bettina Siebers
Universität Duisburg-Essen
J. L. Snoep
Vrije Universiteit Amsterdam
Universiteit Stellenbosch
Hezi Tenenboim
Charité Universitätsmedizin Berlin
B. Teusink
Vrije Universiteit Amsterdam
Spencer J. Williams
University of Melbourne
Mirjam M.C. Wamelink
Universiteit Van Amsterdam
M. Ralser
Max-Planck-Gesellschaft
University of Oxford
Biological Reviews
1464-7931 (ISSN)
Vol. In PressÄmneskategorier (SSIF 2025)
Molekylärbiologi
Cancer och onkologi
DOI
10.1111/brv.70104
PubMed
41319207