Lysine analogue-driven adaptive evolution produces lysine-overproducing wine yeast strains
Artikel i vetenskaplig tidskrift, 2026
Lysine, though a poor nitrogen source for Saccharomyces cerevisiae, is rapidly consumed during wine fermentation and has been linked to enhanced oxidative and sulfur dioxide resistance. To investigate and harness this phenotype, the industrial wine strain P14 was subjected to adaptive laboratory evolution under increasing concentrations of the toxic lysine analogue S-aminoethyl cysteine (AEC). The resulting evolved isolates accumulated up to tenfold more intracellular lysine, displayed elevated reduced glutathione, and exhibited substantially decreased ROS levels while maintaining robust fermentation performance. Whole-genome sequencing revealed extensive genomic rearrangements and recurrent mutations associated with lysine biosynthesis. Among these, the LYS21V127F mutation emerged as the major driver of lysine overproduction and AEC resistance. CRISPR/Cas9 reconstruction of this allele in multiple wine yeasts confirmed its causal role and conferred a competitive advantage during fermentation. These findings demonstrate the effectiveness of AEC-driven evolution for generating lysine-overproducing wine yeasts and highlight LYS21 feedback-resistance as a key metabolic innovation.
Adaptive laboratory evolution (ALE)
Wine fermentation
Glutathione metabolism
Genome evolution
Lysine overproduction
Homocitrate synthase (LYS21)
Författare
Sara Muñiz Calvo
Chalmers, Life sciences, Systembiologi
Consejo Superior de Investigaciones Científicas (CSIC)
Elena Valera-García
Consejo Superior de Investigaciones Científicas (CSIC)
Judit Pardo
Consejo Superior de Investigaciones Científicas (CSIC)
María Vázquez
Consejo Superior de Investigaciones Científicas (CSIC)
Andrés Planells-Cárcel
Consejo Superior de Investigaciones Científicas (CSIC)
Javier Alonso-del-Real
Consejo Superior de Investigaciones Científicas (CSIC)
Ying Su
Northwest A&F university
José María Heras
Lallemand Sas
Anne Ortiz-Julien
Lallemand Sas
Estéfani García-Ríos
Universidad de La Rioja
José Manuel Guillamón
Consejo Superior de Investigaciones Científicas (CSIC)
Food Research International
0963-9969 (ISSN) 18737145 (eISSN)
Vol. 245 120791Ämneskategorier (SSIF 2025)
Evolutionsbiologi
Livsmedelsbioteknik
Genetik och genomik
DOI
10.1016/j.foodres.2026.120791
Relaterade dataset
Dataset [dataset]
URI: http://hdl.handle.net/10261/403811