Evaluation of reference genes for studies of gene expression in human adipose tissue.
Artikel i vetenskaplig tidskrift, 2005

OBJECTIVE: The aim of this study was to evaluate reference genes for expression studies of human adipose tissue. RESEARCH METHODS AND PROCEDURES: Using 52 human adipose tissue expression profiles (HU95), 10 putative reference genes with the lowest variation in expression levels were selected for further studies. Expression stability of these 10 novel and 5 previously established reference genes was evaluated by real-time reverse transcriptase-polymerase chain reaction analysis. For this purpose, 44 adipose tissue biopsies from 27 subjects were chosen to include a wide range of parameters such as sex, age, BMI, depot origin, biopsy procedure, and effects of nutrition. RESULTS: LRP10 was identified as the gene with the least variation in expression levels. The frequently used reference genes RPLP0, 18S rRNA, PPIA, ACTB, and GAPD were ranked as 4, 6, 7, 8, and 10, respectively. DISCUSSION: Our results suggest that LRP10 is a better choice as reference for expression studies of human adipose tissue compared with the most frequently used reference genes.

genetics

Male

Adipose Tissue

Aging

methods

Membrane Transport Proteins

Humans

Adult

Receptors

LDL

Body Mass Index

Reverse Transcriptase Polymerase Chain Reaction

genetics

Female

Gene Expression

LDL-Receptor Related Proteins

Sex Characteristics

Middle Aged

metabolism

Gene Expression Profiling

Oligonucleotide Array Sequence Analysis

Biopsy

Författare

Britt Gabrielsson

Göteborgs universitet

Louise Olofsson

Göteborgs universitet

Anders Sjögren

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

Margareta Jernås

Göteborgs universitet

Anna Elander

Göteborgs universitet

Malin Lönn

Göteborgs universitet

Mats Rudemo

Chalmers, Matematiska vetenskaper, matematisk statistik

Göteborgs universitet

Lena M S Carlsson

Göteborgs universitet

Obesity Research

1071-7323 (ISSN) 1550-8528 (eISSN)

Vol. 13 4 649-52

Ämneskategorier

Endokrinologi och diabetes

Fysiologi

Medicinsk genetik

PubMed

15897472

Mer information

Skapat

2017-10-06