Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary
Artikel i vetenskaplig tidskrift, 2007

A549 cells were briefly exposed to Thioglo-1, which converts thiols to fluorescent adducts. The fluorescent cells were exposed to short (50-300 ms) electric field pulses (500 V across a 15 cm capillary) created at the tip of an electrolyte-filled capillary. Fluorescence microscopy revealed varying degrees of cell permeabilization depending on the conditions. Longer pulses and a shorter cell-capillary tip distance led to a greater decrease in the cell's fluorescence. Live/dead (calcein AM and propidium iodide) testing revealed that a certain fraction of cells died. Longer pulses and shorter cell-capillary tip distances were more deadly. An optimum condition exists at a cell-capillary tip distance of 3.5-4.5 mu m and a pulse duration of 120-150 ms. At these conditions, > 90% of the cells are permeabilized and 80-90% survive.


A. Agarwal

University of Pittsburgh

I. Zudans

University of Pittsburgh

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

S. G. Weber

University of Pittsburgh

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 79 161-167