Evolutionary aspects of the genomic organization of rat chromosome 10.
Artikel i vetenskaplig tidskrift, 2002

Using FISH and RH mapping a chromosomal map of rat chromosome 10 (RNO10) was constructed. Our mapping data were complemented by other published data and the final map was compared to maps of mouse and human chromosomes. RNO10 contained segments homologous to mouse chromosomes (MMU) 11, 16 and 17, with evolutionary breakpoints between the three segments situated in the proximal part of RNO10. Near one of these breakpoints (between MMU17 and 11) we found evidence for an inversion ancestral to the mouse that was not ancestral to the condition in the rat. Within each of the chromosome segments identified, the gene order appeared to be largely conserved. This conservation was particularly clear in the long MMU11-homologous segment. RNO10 also contained segments homologous to three human chromosomes (HSA5, 16, 17). However, within each segment of conserved synteny were signs of more extensive rearrangements. At least 13 different evolutionary breakpoints were indicated in the rat-human comparison. In contrast to what was found between rat and mouse, the rat-human evolutionary breaks were distributed along the entire length of RNO10.

Animals

Rats

Base Sequence

DNA Primers

Polymerase Chain Reaction

Genetic Markers

Molecular Sequence Data

Chromosome Mapping

Fluorescence

genetics

Evolution

In Situ Hybridization

Nucleic Acid Hybridization

Författare

Afrouz Behboudi

Göteborgs universitet

Eleonor Sjöstrand

Göteborgs universitet

Pedro M Gómez-Fabre

Göteborgs universitet

Åsa Sjöling

Göteborgs universitet

Karin Klinga-Levan

Göteborgs universitet

Fredrik Ståhl

Göteborgs universitet

Göran Levan

Göteborgs universitet

Cytogenetic and Genome Research

1424-8581 (ISSN) 1424-859X (eISSN)

Vol. 96 52-9

Ämneskategorier

Genetik

PubMed

12438780