Comparative proteomics of industrial lager yeast reveals differential expression of the cerevisiae and non-cerevisiae parts of their genomes
Artikel i vetenskaplig tidskrift, 2007

The proteomes of three industrial lager beer strains, CMBS33, OG2252 and A15, were analysed under standardised laboratory growth conditions. Protein spots in the 2-DE pattern of the lager strains were subjected to MS/MS to identify protein variants. We found the protein composition of the three lager strains to be qualitatively rather similar, while being substantially different from the Saccharomyces cerevisiae strain BY4742. Database searches using several fully sequenced genomes from the Saccharomyces genera indicated that the non-cerevisiae proteins in the 2-D pattern of lager strains were most closely related to S. bayanus. For many proteins the regulation of the bayanus-like protein and its cerevisiae counterpart varied in a strain-dependent manner, e.g. the bayanus-like form of Tdh3p was roughly eight-fold more abundant than the cerevisiae form in the OG2252 strain. We also found differential regulation of cerevisiae- and bayanus-like proteins during various stress conditions like low temperature growth, and adaptation to high temperatures or high salinity, e.g. for Arg1p, Sti1p and Pdc1p. Our data on the differential regulation of the two genomes in these hybrid strains may have important industrial implications for strain improvement and strain protection. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.








n-terminal acetyltransferases




Robert Caesar

Göteborgs universitet

Johan Palmfeldt

Göteborgs universitet

John Gustafsson

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

Ellinor Pettersson

Göteborgs universitet

Sayed Hossein Hashemi

Göteborgs universitet

Anders Blomberg

Göteborgs universitet


1615-9853 (ISSN) 1615-9861 (eISSN)

Vol. 7 22 4135-4147


Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)