Microfluidic gradient-generating device for pharmacological profiling
Artikel i vetenskaplig tidskrift, 2005

We describe an on-chip microfluidic gradient-generating device that generates concentration gradients spanning nearly 5 orders of magnitude starting from a single concentration. The exiting stream of drugs held at different concentrations remains laminar in a recording chamber and can be presented as 24 discrete solutions to a cell-based sensor. The high-performance characteristics of the device are demonstrated by pharmacological screening of voltage-gated K + channels (hERG) and ligand-gated GABAA receptors using scanning-probe patch-clamp measurements. Multiple data point dose-response curves and IC 50 and EC 50 values were rapidly obtained, typically in less than 30 min, through its combined functionality of gradient generation and open-volume laminar flow. The device facilitates rapid pharmacological profiling of ion channel and GPCR effectors and enables the acquisition of large numbers of data points with minute sample consumption and handling. © 2005 American Chemical Society.

cells

systems

poly(dimethylsiloxane)

channel

xenopus oocytes

drug discovery

receptors

Författare

Johan Pihl

Chalmers University of Technology

J. Sinclair

Chalmers University of Technology

Eskil Sahlin

Göteborgs universitet

Mattias Karlsson

Cellectricon AB

F. Petterson

Cellectricon AB

Jessica Olofsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 77 3897-3903

Ämneskategorier

Analytisk kemi

DOI

10.1021/ac050218+