BOXTO as a real-time thermal cycling reporter dye
Artikel i vetenskaplig tidskrift, 2007

The unsymmetrical cyanine dyes BOXTO (4-[6-(benzoxazole-2-yl-(3-methyl-)-2,3-dihydro-(benzo-1,3-thiazole)-2me thylidene)]-1-methyl-quinolinium chloride) and its positive divalent derivative BOXTO-PRO (4-[(3-methyl-6(benzoxazole-2-yl)-2,3-dihydro-(benzo-1,3-thiazole)-2-met hylidene)]-1-(3-trimethylammonium-propyl)-quinolinium dibromide) were studied as real-time PCR reporting fluorescent dyes and compared to SYBR GREEN I (SG) (2-[N-(3-dimethylaminopropyl)-N-propylamino]-4-[2,3-dihydro-3-methyl-(be nzo-1,3-thiazol-2-yl)-methylidene]-1-phenyl- quinolinium). Unmodified BOXTO showed no inhibitory effects on real-time PCR, while BOXTO-PRO showed complete inhibition. Sufficient fluorescent signal was acquired when 0.5-1.0 mu M BOXTO was used with RotorGene and iCycler platforms. Statistical analysis showed that there is no significant difference between the efficiency and dynamic range of BOXTO and SG. BOXTO stock solution (1.5 mM) was stable at -20 degrees C for more than one year and 40 mu M BOXTO solution was more stable than 5x SG when both were stored at 4 degrees C for 45 days.

QUANTIFICATION

SUBNANOSECOND RELAXATION DYNAMICS

BOXTO-PRO

DNA-BINDING

PCR

BOXTO

GROOVE

real-time PCR

PHOTOPHYSICS

GREEN-I

unsymmetrical cyanine dyes

UNSYMMETRICAL CYANINE DYES

AMPLIFICATION

Författare

Ashraf Ahmad

Chalmers, Kemi- och bioteknik

Journal of Biosciences

0250-5991 (ISSN)

Vol. 32 229-239

Ämneskategorier

Kemiteknik