Effect of shear stress on the expression of coagulation and fibrinolytic factors in both smooth muscle and endothelial cells in a co-culture model.
Artikel i vetenskaplig tidskrift, 2008

Blood vessels are subjected to forces due to the flow. Endothelial cells (EC) are recipients, cross-talk with smooth muscle cells (SMC), and regulate physiology. It was hypothesized that both EC and SMC respond to shear stress, which alters the expression of factors in coagulation and fibrinolysis. METHODS: A co-culture of human saphenous vein EC (HSVEC) and human saphenous vein SMC (HSVSMC) was exposed to shear, following which the cells were separated. Gene expression of tissue factor, thrombomodulin (TM), plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) were analyzed with real-time RT-PCR. Protein expression was studied with ELISA. In HSVEC, the expression of PAI-1 (x2.1), tPA (x1.8), uPA (x1.6), tissue factor (x2.5) and TM (x1.9) was upregulated after 4 h of shear compared to controls. After 24 h of shear, expression was still upregulated in tPA (x2.3) and TM (x1.6). In HSVSMC, change in expression of PAI-1 (x2.1) was present after 4 h and in uPA (x2.1), and TM (x0.4) after 24 h. Both HSVEC and HSVSMC responded to shear, which led to altered expression of coagulation and fibrinolytic factors. This indicates that SMC, and interactions between EC and SMC, are more important in the regulation of vascular wall hemostasis than earlier studies have reported.

Humans

metabolism

metabolism

Stress

Endothelial Cells

Plasminogen Activator Inhibitor 1

Coculture Techniques

Myocytes

Urinary Plasminogen Activator

Blood Coagulation

Cells

Smooth Muscle

metabolism

metabolism

metabolism

Thrombomodulin

Tissue Plasminogen Activator

physiology

metabolism

Cultured

Thromboplastin

Mechanical

metabolism

Författare

Gisela Helenius

Göteborgs universitet

Sepideh Heydarkhan-Hagvall

Göteborgs universitet

Maricris Esguerra

Göteborgs universitet

Helen Fink

Göteborgs universitet

Rigmor Söderberg

Göteborgs universitet

Bo Risberg

Göteborgs universitet

European Surgical Research

0014-312X (ISSN) 1421-9921 (eISSN)

Vol. 40 325-32

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1159/000118028

PubMed

18303268