Neither Notch1 expression nor cellular size correlate with mesenchymal stem cell properties of adult articular chondrocytes.
Artikel i vetenskaplig tidskrift, 2008

BACKGROUND: Tissue repair is thought to be regulated by progenitor cells, which in other tissues are characterized by their Notch1 expression or small cellular size. Here we studied if these traits affect the chondrogenic potential and are markers for multipotent progenitor cell populations in adult articular cartilage. METHODS: Directly isolated articular chondrocytes were sorted with regard to their Notch1 expression or cellular size. Their colony forming efficiency (CFE) and their potential to differentiate towards adipogenic, osteogenic and chondrogenic lineages were investigated. The different sorted populations were also expanded in monolayer and analyzed in the same manner as the directly isolated cells. RESULTS: No differences in CFE or adipogenic, osteogenic and chondrogenic potentials were detected among the sorted populations. Expanded cells displayed a higher osteochondral potential than directly isolated cells. CONCLUSION: Cellular size or Notch1 expression is not per se a specific marker for mesenchymal progenitor cells in adult articular cartilage. Monolayer-expanded adult chondrocytes contain a larger mesenchymal progenitor cell-like population than directly isolated cells, highly likely as a result of dedifferentiation. If there are resident Notch1-positive cells or cells of a specific size in adult articular cartilage with functional features of progenitor cells, the population consists of only a very small number of cells.

Cattle

Flow Cytometry

Cell Size

Adult Stem Cells

Receptor

Mesenchymal Stem Cells

cytology

metabolism

cytology

Cell Differentiation

metabolism

Colony-Forming Units Assay

Notch1

metabolism

Cell Culture Techniques

metabolism

Articular

Osteogenesis

cytology

cytology

Cartilage

metabolism

Immunohistochemistry

Cell Separation

Animals

Adipogenesis

Chondrocytes

Författare

Camilla Karlsson

Göteborgs universitet

Hanna Stenhamre

Göteborgs universitet

Chalmers, Kemi- och bioteknik, Polymerteknologi

Joakim Sandstedt

Göteborgs universitet

Anders Lindahl

Göteborgs universitet

Cells Tissues Organs

1422-6405 (ISSN) 1422-6421 (eISSN)

Vol. 187 4 275-85

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi

Cell- och molekylärbiologi

DOI

10.1159/000113409

PubMed

18187938