A network-based analysis of allergen-challenged CD4+T cells from patients with allergic rhinitis
Artikel i vetenskaplig tidskrift, 2006

We performed a network-based analysis of DNA microarray data from allergen-challenged CD4 + T cells from patients with seasonal allergic rhinitis. Differentially expressed genes were organized into a functionally annotated network using the Ingenuity Knowledge Database, which is based on manual review of more than 200000 publications. The main function of this network is the regulation of lymphocyte apoptosis, a role associated with several genes of the tuber necrosis factor superfamily. The expression of TNFRSF4, one of the genes in this family, was found to be 48 times higher in allergen-challenged cells than in diluent-challenged cells. TNFRSF4 is known to inhibit apoptosis and to enhance Th2 proliferation. Examination of a different material of allergen-stimulated peripheral blood mononuclear cells showed a higher number of interleukin-4 + type 2 CD4 + T (Th2) cells in patients than in controls (P < 0.01), as well as a higher number of non-apoptotic Th2 cells in patients (P < 0.01). The number of Th2 cells expressing TNFRSF4, TNFSF7 and TNFRSF1B was also significantly higher in patients. Treatment with anti-TNFSF4 resulted in a significantly decreased number of Th2 cells (P < 0.05). A logical inference from all this is that the proliferation of allergen-challenged Th2 cells is associated with a decreased apoptosis of Th2 cells and an increase in TNFRSF4 signalling.

airway disease

costimulatory molecules

immune-responses

t-cells

atopic-dermatitis

ox40 ligand

receptor expression

inflammation

t-helper-2 cells

th2

Författare

Mikael Benson

Göteborgs universitet

Lena M S Carlsson

Göteborgs universitet

Gilles Guillot

INRA Institut National de La Recherche Agronomique

Chalmers University of Technology

Margareta Jernås

Göteborgs universitet

M.A. Langston

University of Tennessee

Mats Rudemo

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

Bengt Andersson

Göteborgs universitet

Genes and Immunity

1466-4879 (ISSN) 1476-5470 (eISSN)

Vol. 7 514-521

Ämneskategorier

Immunologi inom det medicinska området

Genetik

DOI

10.1038/sj.gene.6364322