Fixation of Functionalized DNA Nanostructures
Licentiatavhandling, 2009

This thesis features results from two different studies on a fully addressable non-repetitive network on the nanometre scale, assembled by synthetic DNA molecules. A bio-inspired molecular approach, based on principles of self-assembly, has brought about a paradigm shift in nanoscience, from a top-down to a bottom-up perspective. The studied system in this thesis is a two-dimensional assembly of hexagonal unit cells with the potential of controlling diverse processes, such as energy transfer or chemical synthesis, at the highest precision. The first study proves successful formation of the first generation of this network, a fused bihexagonal structure. The study also illustrates a method of addressing a specific location in the system using triplex recognition. Furthermore, the triplex recognition functions in principle as an information storage device. The second study introduces a fixation strategy based on the principles of click chemistry. By covalently cross-linking a DNA hexagon the stability is markedly increased, creating a module that may readily be adapted as a building block for larger structures.

triplex recognition

dna

self-assembly

gel electrophoresis

nanotechnology

cross-linking

spectroscopy

fixation

fret

click chemistry

10:an, Kemihuset, Chalmers tekniska högskola

Författare

Erik Lundberg

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Ämneskategorier

Kemiteknik

Licentiatuppsatser vid Institutionen för kemi- och bioteknik, Chalmers tekniska högskola

10:an, Kemihuset, Chalmers tekniska högskola