Proteomic characterization of IgY preparations purified with a water dilution method.
Artikel i vetenskaplig tidskrift, 2008
Antigen-specific chicken IgY antibodies have been used for oral immunotherapy as an alternative or complement to antibiotics in several studies. The water dilution (WD) method has several advantages for purifying IgY. It is rapid, efficient, suitable for large-scale production, and nothing but water is added. The water-soluble fraction contains other proteins and lipids besides IgY. The protein content was characterized by two-dimensional gel electrophoresis (2DGE) and nanoflow liquid chromatography coupled offline to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (nanoLC-MALDI TOF/TOF MS). Protein analysis was complicated due to the large dynamic concentration range, but 26 proteins could be identified. The relative protein concentrations in different batches were very similar according to protein patterns on 1D gels and protein concentration determinations. Thus, the purification method has a high reproducibility. The concentrations of cholesterols and triglycerides were low and should not have an effect on the plasma levels of treated patients. Purification of IgY for oral use with WD is therefore a recommended method.
chemistry
analysis
Egg Yolk
Lipids
Chickens
Matrix-Assisted Laser Desorption-Ionization
Egg Proteins
Immunoglobulins
Spectrometry
Water
Two-Dimensional
Proteomics
chemistry
isolation & purification
isolation & purification
Electrophoresis
Animals
chemistry
Mass
Gel
Författare
Elin Nilsson
Jörg Hanrieder
Göteborgs universitet
Jonas Bergquist
Anders Larsson
Journal of Agricultural and Food Chemistry
0021-8561 (ISSN) 1520-5118 (eISSN)
Vol. 56 24 11638-42Ämneskategorier
Biokemi och molekylärbiologi
Analytisk kemi
Livsmedelsvetenskap
Biologiska vetenskaper
Immunologi inom det medicinska området
Näringslära
DOI
10.1021/jf802626t
PubMed
19053374