The direction of human mesenchymal stem cells into the chondrogenic lineage is influenced by the features of hydrogel carriers
Artikel i vetenskaplig tidskrift, 2017
Low back pain is a major public health issue in the Western world, one main cause is believed to be intervertebral disc (IVD) degeneration. To halt/diminish IVD degeneration, cell therapy using different biomaterials e.g. hydrogels as cell carriers has been suggested. In this study, two different hydrogels were examined (in vitro) as potential cell carriers for human mesenchymal stem cells (hMSCs) intended for IVD transplantation. The aim was to investigate cell- survival and chondrogenic differentiation of hMSCs when cultured in hydrogels Puramatrix((R)) or Hydromatrix((R)) and potential effects of stimulation with growth hormone (GH). hMSCs/hydrogel cultures were investigated for cell-viability, attachment, gene expressionof chondrogenic markers SOX9, COL2A1, ACAN and accumulation of extracellular matrix (ECM). In both hydrogel types, hMSCs were viable for 28 days, expressed integrin beta 1 which indicates adhesion of hMSCs. Differentiation was observed into chondrocyte-like cells, in a higher extent in hMSCs/Hydromatrix((R)) cultures when compared to hMSCs/Puramatrix ((R)) hydrogel cultures. Gene expression analyses of chondrogenic markers verified results. hMSCs/hydrogel cultures stimulated with GH displayed no significant effects on chondrogenesis. In conclusion, both hydrogels, especially Hydromatrix((R)) was demonstrated as a promising cell carrier in vitro for hMSCs, when directed into chondrogenesis. This knowledge could be useful in biological approaches for regeneration of degenerated human IVDs.
Cell carrier
vivo
repair
Hydrogels
transplantation
intervertebral disc degeneration
applications
Disc degeneration
ott j. e.
in-vitro
tissue-engineering
model
Intervertebral disc
p73
1964
growth-factors
Anatomy & Morphology
Human mesenchymal stem cells
Cell Biology
v4
low-back-pain
histochemie
therapy
Författare
A. Hansson
Göteborgs universitet
Anna Wenger
Chalmers, Biologi och bioteknik
H. B. Henriksson
Göteborgs universitet
Sahlgrenska universitetssjukhuset
S. Li
Göteborgs universitet
B. R. Johansson
Göteborgs universitet
H. Brisby
Sahlgrenska universitetssjukhuset
Göteborgs universitet
Tissue and Cell
0040-8166 (ISSN) 15323072 (eISSN)
Vol. 49 1 35-44Ämneskategorier
Cell- och molekylärbiologi
Styrkeområden
Livsvetenskaper och teknik (2010-2018)
DOI
10.1016/j.tice.2016.12.004