Electrospray sample injection for single-particle imaging with x-ray lasers
Artikel i vetenskaplig tidskrift, 2019
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
Författare
Johan Bielecki
European XFEL
Uppsala universitet
Max F. Hantke
Refeyn Ltd.
University of Oxford
Uppsala universitet
Benedikt J. Daurer
Uppsala universitet
NUS Centre for Bioimaging Sciences
Hemanth K.N. Reddy
Uppsala universitet
Dirk Hasse
Uppsala universitet
Daniel S.D. Larsson
Uppsala universitet
Laura H. Gunn
Uppsala universitet
Martin Svenda
Alba Nova Universitetscentrum
Uppsala universitet
Anna Munke
Uppsala universitet
Jonas A. Sellberg
Alba Nova Universitetscentrum
Uppsala universitet
Leonie Flueckiger
La Trobe University
Alberto Pietrini
Uppsala universitet
Carl Nettelblad
Uppsala universitet
Ida Lundholm
Uppsala universitet
Gunilla Carlsson
Uppsala universitet
Kenta Okamoto
Uppsala universitet
N. Timneanu
Uppsala universitet
Daniel Westphal
Uppsala universitet
Olena Kulyk
Czech Academy of Sciences
Akifumi Higashiura
Osaka University
Hiroshima University
Gijs Van Der Schot
Bijvoet Center for Biomolecular Research
Uppsala universitet
Ne Te Duane Loh
NUS Centre for Bioimaging Sciences
Universiti Kebangsaan Singapura (NUS)
Taylor E. Wysong
Stanford University
Christoph Bostedt
Argonne National Laboratory
Northwestern University
Tais Gorkhover
Stanford University
Bianca Iwan
Leibniz Universität Hannover
Stanford University
M. Marvin Seibert
Uppsala universitet
Timur Osipov
Stanford University
Peter Walter
Stanford University
Philip Hart
Stanford University
Max Bucher
Argonne National Laboratory
Anatoli Ulmer
Technische Universität Berlin
Dipanwita Ray
Stanford University
Gabriella Carini
Brookhaven National Laboratory
Stanford University
Ken Ferguson
Stanford University
Inger Andersson
Uppsala universitet
Jakob Andreasson
Chalmers, Fysik, Materialfysik
Czech Academy of Sciences
Janos Hajdu
Czech Academy of Sciences
Uppsala universitet
Filipe R.N.C. Maia
Uppsala universitet
Lawrence Berkeley National Laboratory
Science advances
2375-2548 (eISSN)
Vol. 5 5 eaav8801Ämneskategorier
Analytisk kemi
Atom- och molekylfysik och optik
Annan fysik
DOI
10.1126/sciadv.aav8801