Aborting meiosis overcomes hybrid sterility
Preprint, 2020
Hybrids between species or diverged lineages contain fundamentally novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explored to what extent and how an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 yeast diploid backgrounds with different genomic structures and levels of sterility. Genome analyses of 284 clones revealed that RTG promoted recombination and generated extensive regions of loss-ofheterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination was reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially occurred in regions with local sequence homology and in meiotic recombination hotspots. The loss-of-heterozygosity had a profound impact on sexual and asexual fitness, and enabled genetic mapping of phenotypic differences in sterile lineages where linkage or association analyses failed. We propose that RTG gives sterile hybrids access to a natural route for genome recombination and adaptation.
Författare
Simone Mozzachiodi
Université Côte d'Azur
Lorenzo Tattini
Université Côte d'Azur
Agnès Llored
Université Côte d'Azur
Agurtzane Irizar
Université Côte d'Azur
Neža Škofljanc
Université Côte d'Azur
Melania D’Angiolo
Université Côte d'Azur
Matteo De Chiara
Université Côte d'Azur
Benjamin P. Barré
Université Côte d'Azur
Jia Xing Yue
Université Côte d'Azur
Angela Lutazi
Université Côte d'Azur
Sophie Loeillet
Université de recherche Paris Sciences et Lettres
Raphaelle Laureau
Université de recherche Paris Sciences et Lettres
Souhir Marsit
Université de recherche Paris Sciences et Lettres
Université de Montpellier
Simon Stenberg
Göteborgs universitet
Benoit Albaud
Institut Curie
Karl Persson
Göteborgs universitet
Jean-Luc Legras
Université de Montpellier
Sylvie Dequin
Université de Montpellier
Jonas Warringer
Göteborgs universitet
Alain Nicolas
Université de recherche Paris Sciences et Lettres
Gianni Liti
Université Côte d'Azur
Ämneskategorier
Evolutionsbiologi
Biokemi och molekylärbiologi
Mikrobiologi
Bioinformatik och systembiologi
Genetik
DOI
10.1101/2020.12.04.411579