Optical Biosensors Towards Point of Care Testing of Various Biochemicals
Kapitel i bok, 2022

A steady rise in the use of point-of-care devices is warranted by the growing demand for medical attention and increased use of portable biosensing devices across various industries in different domains. Portable point-of-care devices provide simple, cost- and time-effective, reliable detection of various chemicals such as biomolecules, toxins or environmental pollutants, and microscopic entities such as parasites, viruses, bacteria and other pathogens. Among different biosensors, optical biosensors offer high sensitivity in the range of 10−6–10−8 RIU, real-time monitoring capability, cost-effectiveness, rapidity and compatibility to miniaturization. The versatility of this class of biosensors makes them attractive candidates, holding promising potential for use as next-generation point-of-care testing devices. Optical biosensors use optical field parameters such as the amplitude, frequency, phase and polarization state to probe molecular interactions. Optical biosensors can be classified into label-based (e.g. fluorescence) and label-free (e.g. surface plasmon resonance) biosensors. Advances in the optical biosensing domain towards integrated optics, integration of microfluidic technology and microelectromechanical systems-assisted sensor fabrication techniques have significantly contributed to the field as they facilitate the fabrication and development of portable, cost-effective and high-throughput optical biosensing device. Label-based techniques offer excellent sensitivity and specificity for interrogating biomolecular interactions. Whereas label-free techniques directly detect and interpret the changes in optical parameters of the incident light wave on the biomolecules embedded on sensor surfaces, without hindered by photo-bleaching or other limitations faced by marker-dependent approaches. This chapter provides a brief overview of currently available label-based and label-free biosensors and discusses the potential and key limitations that require further advances in the field to facilitate successful commercialization of these techniques.

Point-of-care devices

Fluorescence-based biosensors

Interferometry

Optical biosensors

SPR

Evanescence-based biosensors

Smart-phone-based PoC

Fibre-optic biosensors

Författare

Vinoth Sundar Rajan

Chalmers, Biologi och bioteknik, Kemisk biologi

Archana Ramadoss

Nanolane

Materials Horizons: From Nature to Nanomaterials

25245384 (ISSN) 25245392 (eISSN)

245-277

Ämneskategorier

Medicinsk laboratorie- och mätteknik

Atom- och molekylfysik och optik

Annan fysik

DOI

10.1007/978-981-16-3645-5_11

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Senast uppdaterat

2023-04-21