Effect of shear stress on the expression of coagulation and fibrinolytic factors in both smooth muscle and endothelial cells in a co-culture model.
Artikel i vetenskaplig tidskrift, 2008
Blood vessels are subjected to forces due to the flow. Endothelial cells (EC) are recipients, cross-talk with smooth muscle cells (SMC), and regulate physiology. It was hypothesized that both EC and SMC respond to shear stress, which alters the expression of factors in coagulation and fibrinolysis. METHODS: A co-culture of human saphenous vein EC (HSVEC) and human saphenous vein SMC (HSVSMC) was exposed to shear, following which the cells were separated. Gene expression of tissue factor, thrombomodulin (TM), plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) were analyzed with real-time RT-PCR. Protein expression was studied with ELISA. In HSVEC, the expression of PAI-1 (x2.1), tPA (x1.8), uPA (x1.6), tissue factor (x2.5) and TM (x1.9) was upregulated after 4 h of shear compared to controls. After 24 h of shear, expression was still upregulated in tPA (x2.3) and TM (x1.6). In HSVSMC, change in expression of PAI-1 (x2.1) was present after 4 h and in uPA (x2.1), and TM (x0.4) after 24 h. Both HSVEC and HSVSMC responded to shear, which led to altered expression of coagulation and fibrinolytic factors. This indicates that SMC, and interactions between EC and SMC, are more important in the regulation of vascular wall hemostasis than earlier studies have reported.
Humans
metabolism
metabolism
Stress
Endothelial Cells
Plasminogen Activator Inhibitor 1
Coculture Techniques
Myocytes
Urinary Plasminogen Activator
Blood Coagulation
Cells
Smooth Muscle
metabolism
metabolism
metabolism
Thrombomodulin
Tissue Plasminogen Activator
physiology
metabolism
Cultured
Thromboplastin
Mechanical
metabolism
Författare
Gisela Helenius
Göteborgs universitet
Sepideh Heydarkhan-Hagvall
Göteborgs universitet
Maricris Esguerra
Göteborgs universitet
Helen Fink
Göteborgs universitet
Rigmor Söderberg
Göteborgs universitet
Bo Risberg
Göteborgs universitet
European Surgical Research
0014-312X (ISSN) 1421-9921 (eISSN)
Vol. 40 4 325-32Ämneskategorier
MEDICIN OCH HÄLSOVETENSKAP
DOI
10.1159/000118028
PubMed
18303268