CRISPR-based mutation detection using graphene chip enables cancer detection

Research Project, 2021 – 2023

Purpose and goal: The project goal is to develop a powerful and simple diagnostic graphene chip for fast and cost-effective mutation detection in cancer diagnostics. Our graphene-based chip will be a minimally invasive, sensitive, simple, fast and cost-effective method for detecting circulating tumor DNA (ctDNA).
Expected results and effects: CRISPR / Cas9 will be used for the detection of circulating tumor DNA (ctDNA) via mutation assays in fluid biopsies with extreme precision and reliability. A single miniature chip (1 cm2) contains several hundred different detection areas where each area can be produced for individual mutations. Since the chip is made of recyclable material (graphene and biodegradable plastic), this technological environmental footprint will be minimal. Approach and implementation: The Cas9 detection module will be coupled to an advanced 2D graphene based material that will form the signal generating part of the chip. As ctDNA binds to Cas9, the electrical conductivity of the graphene chip will change, generating a specific measurement signal. The chip can be connected to a simple electronic reading device via a USB socket and can be used without additional advanced equipment.