CRISPR-Cas target recognition for sensing viral and cancer biomarkers
Review article, 2024

Nucleic acid-based diagnostics is a promising venue for detection of pathogens causing infectious diseases and mutations related to cancer. However, this type of diagnostics still faces certain challenges, and there is a need for more robust, simple and cost-effective methods. Clustered regularly interspaced short palindromic repeats (CRISPRs), the adaptive immune systems present in the prokaryotes, has recently been developed for specific detection of nucleic acids. In this review, structural and functional differences of CRISPR-Cas proteins Cas9, Cas12 and Cas13 are outlined. Thereafter, recent reports about applications of these Cas proteins for detection of viral genomes and cancer biomarkers are discussed. Further, we highlight the challenges associated with using these technologies to replace the current diagnostic approaches and outline the points that need to be considered for designing an ideal Cas-based detection system for nucleic acids. Graphical Abstract

Author

Shadi Rahimi

Chalmers, Life Sciences, Systems and Synthetic Biology

Sri Renukadevi Balusamy

Sejong Univ

Haribalan Perumalsamy

Hanyang University

Anders Stahlberg

University of Gothenburg

Ivan Mijakovic

Chalmers, Life Sciences, Systems and Synthetic Biology

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 52 17 10040-10067

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Subject Categories

Cell and Molecular Biology

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1093/nar/gkae736

PubMed

39189452

More information

Latest update

10/7/2024