Genome-wide analysis of maltose utilization and regulation in aspergilli
Journal article, 2009

Maltose utilization and regulation in aspergilli is of great importance for cellular physiology and industrial fermentation processes. In Aspergillus oryzae, maltose utilization requires a functional MAL locus, each composed of three genes: MALR encoding a regulatory protein, MALT encoding maltose permease and MALS encoding maltase. Through a comparative genome and transcriptome analysis we show that the MAL regulon system is active in A. oryzae while it is not present in Aspergillus niger. In order to utilize maltose, A. niger requires a different regulatory system that involves the AmyR regulator for glucoamylase (glaA) induction. Analysis of reporter metabolites and subnetworks illustrate the major route of maltose transport and metabolism in A. oryzae. This demonstrates that overall metabolic responses of A. oryzae occur in terms of genes, enzymes, and metabolites when altering the carbon source. Although the amount of knowledge on maltose transport and metabolism is far from being complete in Aspergillus spp., our study not only helps to understand the sugar preference in industrial fermentation processes, but also indicates how maltose affects gene expression and overall metabolism.

Carbon sources

Transcriptome

Genome

Aspergilli

Author

Wanwipa Vongsangnak

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Margarita Salazar Pena

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Kim Hansen

Novozymes A/S

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Microbiology

1350-0872 (ISSN) 1465-2080 (eISSN)

Vol. 155 12 3893-3902

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.1099/mic.0.031104-0

PubMed

19696104

More information

Latest update

7/3/2018 1