Genome-wide analysis of maltose utilization and regulation in aspergilli
Artikel i vetenskaplig tidskrift, 2009
Maltose utilization and regulation in aspergilli is of great importance
for cellular physiology and industrial fermentation processes.
In Aspergillus oryzae, maltose utilization requires a functional
MAL locus, each composed of three genes: MALR encoding a regulatory
protein, MALT encoding maltose permease and MALS encoding
maltase. Through a comparative genome and transcriptome
analysis we show that the MAL regulon system is active in A.
oryzae while it is not present in Aspergillus niger. In order to utilize
maltose, A. niger requires a different regulatory system that
involves the AmyR regulator for glucoamylase (glaA) induction.
Analysis of reporter metabolites and subnetworks illustrate the
major route of maltose transport and metabolism in A. oryzae. This
demonstrates that overall metabolic responses of A. oryzae occur in
terms of genes, enzymes, and metabolites when altering the carbon
source. Although the amount of knowledge on maltose transport
and metabolism is far from being complete in Aspergillus spp., our
study not only helps to understand the sugar preference in industrial
fermentation processes, but also indicates how maltose affects
gene expression and overall metabolism.