Integrative model of the response of yeast to osmotic shock
Journal article, 2005

Integration of experimental studies with mathematical modeling allows insight into systems properties, prediction of perturbation effects and generation of hypotheses for further research. We present a comprehensive mathematical description of the cellular response of yeast to hyperosmotic shock. The model integrates a biochemical reaction network comprising receptor stimulation, mitogen-activated protein kinase cascade dynamics, activation of gene expression and adaptation of cellular metabolism with a thermodynamic description of volume regulation and osmotic pressure. Simulations agree well with experimental results obtained under different stress conditions or with specific mutants. The model is predictive since it suggests previously unrecognized features of the system with respect to osmolyte accumulation and feedback control, as confirmed with experiments. The mathematical description presented is a valuable tool for future studies on osmoregulation in yeast and—with appropriate modifications—other organisms. It also serves as a starting point for a comprehensive description of cellular signaling.


Edda Klipp

Max Planck Society

Bodil Nordlander

University of Gothenburg

Roland Kruger

Humboldt University of Berlin

Peter Gennemark

Chalmers, Computer Science and Engineering (Chalmers), Computing Science (Chalmers)

Stefan Hohmann

University of Gothenburg

Nature Biotechnology

1087-0156 (ISSN)

Vol. 23 8 975-82.

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

Computational Mathematics





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