Changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins in patients with critical illness.
Journal article, 2009
Insulin resistance develops rapidly during critical illness. The release of adipokines from adipose tissue is thought to play a key role in the development of insulin resistance, as are elevated levels of acute-phase proteins. The aim of this study was to identify changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins during critical illness. From 8 patients with subarachnoidal hemorrhage, consecutive blood samples and adipose tissue biopsies were obtained at 3 time points, twice during intensive care (1-2 days [IC1] and 7-9 days after subarachnoidal hemorrhage) and once after 8 months (recovery). The patients received a continuous insulin infusion to maintain normal glucose levels reflecting insulin resistance. The DNA microarray analysis showed increased zink-alpha2 glycoprotein (ZAG) and phospholipase A2, group IIA messenger RNA levels during intensive care compared with recovery (P < .05). Real-time polymerase chain reaction confirmed the increased expression of ZAG and phospholipase A2, group IIA. Plasma levels of ZAG, serum amyloid A, and C-reactive protein were higher at 7 to 9 days after subarachnoidal hemorrhage compared with either IC1 or recovery (P = .0001); and plasma levels of retinol-binding protein 4 and adiponectin were lower at IC1 compared with recovery (P = .05). The described changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins may influence the development of insulin resistance during critical illness.
genetics
genetics
RNA
chemistry
Gene Expression Regulation
Acute-Phase Proteins
Oligonucleotide Array Sequence Analysis
physiology
biosynthesis
Adipokines
Adipose Tissue
Critical Illness
therapeutic use
Insulin Resistance
metabolism
metabolism
blood
Blood Glucose
Humans
blood
Subarachnoid Hemorrhage
Middle Aged
physiology
genetics
genetics
genetics
Biopsy
Reverse Transcriptase Polymerase Chain Reaction
Female
Male
Insulin
metabolism
Author
Margareta Jernås
University of Gothenburg
Bob Olsson
University of Gothenburg
Kajsa Sjöholm
University of Gothenburg
Anders Sjögren
Chalmers, Mathematical Sciences, Mathematical Statistics
University of Gothenburg
Mats Rudemo
Chalmers, Mathematical Sciences, Mathematical Statistics
University of Gothenburg
Bengt Nellgård
University of Gothenburg
Lena M S Carlsson
University of Gothenburg
Carl David Sjöström
University of Gothenburg
Metabolism: clinical and experimental
1532-8600 (ISSN)
Vol. 58 1 102-8Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.metabol.2008.08.012
PubMed
19059537