Changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins in patients with critical illness.
Artikel i vetenskaplig tidskrift, 2009

Insulin resistance develops rapidly during critical illness. The release of adipokines from adipose tissue is thought to play a key role in the development of insulin resistance, as are elevated levels of acute-phase proteins. The aim of this study was to identify changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins during critical illness. From 8 patients with subarachnoidal hemorrhage, consecutive blood samples and adipose tissue biopsies were obtained at 3 time points, twice during intensive care (1-2 days [IC1] and 7-9 days after subarachnoidal hemorrhage) and once after 8 months (recovery). The patients received a continuous insulin infusion to maintain normal glucose levels reflecting insulin resistance. The DNA microarray analysis showed increased zink-alpha2 glycoprotein (ZAG) and phospholipase A2, group IIA messenger RNA levels during intensive care compared with recovery (P < .05). Real-time polymerase chain reaction confirmed the increased expression of ZAG and phospholipase A2, group IIA. Plasma levels of ZAG, serum amyloid A, and C-reactive protein were higher at 7 to 9 days after subarachnoidal hemorrhage compared with either IC1 or recovery (P = .0001); and plasma levels of retinol-binding protein 4 and adiponectin were lower at IC1 compared with recovery (P = .05). The described changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins may influence the development of insulin resistance during critical illness.

genetics

genetics

RNA

chemistry

Gene Expression Regulation

Acute-Phase Proteins

Oligonucleotide Array Sequence Analysis

physiology

biosynthesis

Adipokines

Adipose Tissue

Critical Illness

therapeutic use

Insulin Resistance

metabolism

metabolism

blood

Blood Glucose

Humans

blood

Subarachnoid Hemorrhage

Middle Aged

physiology

genetics

genetics

genetics

Biopsy

Reverse Transcriptase Polymerase Chain Reaction

Female

Male

Insulin

metabolism

Författare

Margareta Jernås

Göteborgs universitet

Bob Olsson

Göteborgs universitet

Kajsa Sjöholm

Göteborgs universitet

Anders Sjögren

Chalmers, Matematiska vetenskaper, matematisk statistik

Göteborgs universitet

Mats Rudemo

Chalmers, Matematiska vetenskaper, matematisk statistik

Göteborgs universitet

Bengt Nellgård

Göteborgs universitet

Lena M S Carlsson

Göteborgs universitet

Carl David Sjöström

Göteborgs universitet

Metabolism: clinical and experimental

1532-8600 (ISSN)

Vol. 58 1 102-8

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1016/j.metabol.2008.08.012

PubMed

19059537