Proteomic Analysis of Plasma Membrane Vesicles
Journal article, 2009
(Figure Presented) A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flow-cell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
vesicles
integration
secretory vesicles
receptor
fractionation
cells
immunoglobulin-e
lipids
membrane proteins
plasma membranes
proteins
membrane isolation
vesiculation
proteomics
microparticles
Author
Brigitte Bauer
Chalmers
M. Davidson
Nanoxis Consulting AB
Owe Orwar
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Angewandte Chemie - International Edition
1433-7851 (ISSN) 1521-3773 (eISSN)
Vol. 48 9 1656-1659Subject Categories
Physical Chemistry
Chemical Sciences
DOI
10.1002/anie.200803898