Proteomic Analysis of Plasma Membrane Vesicles
Journal article, 2009

(Figure Presented) A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flow-cell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

immunoglobulin-e

cells

membrane isolation

vesicles

proteomics

lipids

plasma membranes

proteins

fractionation

receptor

vesiculation

membrane proteins

microparticles

secretory vesicles

integration

Author

Brigitte Bauer

Chalmers

M. Davidson

Nanoxis AB

Owe Orwar

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 48 9 1656-1659

Subject Categories

Physical Chemistry

Chemical Sciences

DOI

10.1002/anie.200803898

More information

Latest update

9/10/2018