Proteomic Analysis of Plasma Membrane Vesicles
Artikel i vetenskaplig tidskrift, 2009

(Figure Presented) A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flow-cell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

membrane proteins

microparticles

proteins

membrane isolation

vesicles

plasma membranes

cells

proteomics

fractionation

secretory vesicles

immunoglobulin-e

lipids

receptor

vesiculation

integration

Författare

Brigitte Bauer

Chalmers University of Technology

M. Davidson

Nanoxis AB

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 48 1656-1659

Ämneskategorier

Fysikalisk kemi

Kemi

DOI

10.1002/anie.200803898