Steady-State Electrochemical Determination of Lipidic Nanotube Diameter Utilizing an Artificial Cell Model
Journal article, 2010

By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick’s first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick’s law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters Δi (change in current) and nanotube length. Catechol was used to determine the Δi attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.


Kelly L. Adams

University of Gothenburg

Johan Engelbrektsson

University of Gothenburg

Marina Voinova

University of Gothenburg

Bo Zhang

Pennsylvania State University

University of Washington

Daniel J. Eves

Pennsylvania State University

Roger Karlsson

University of Gothenburg

M. L. Heien

Pennsylvania State University

Ann-Sofie Cans

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Andrew Ewing

University of Gothenburg

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 82 3 1020-1026

Subject Categories

Physical Chemistry

Analytical Chemistry

Condensed Matter Physics



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4/6/2018 1