Global transcriptional response of Saccharomyces cerevisiae to the deletion of SDH3
Journal article, 2009

Background: Mitochondrial respiration is an important and widely conserved cellular function in eukaryotic cells. The succinate dehydrogenase complex (Sdhp) plays an important role in respiration as it connects the mitochondrial respiratory chain to the tricarboxylic acid (TCA) cycle where it catalyzes the oxidation of succinate to fumarate. Cellular response to the Sdhp dysfunction (i.e. impaired respiration) thus has important implications not only for biotechnological applications but also for understanding cellular physiology underlying metabolic diseases such as diabetes. We therefore explored the physiological and transcriptional response of Saccharomyces cerevisiae to the deletion of SDH3, that codes for an essential subunit of the Sdhp. Results: Although the Sdhp has no direct role in transcriptional regulation and the flux through the corresponding reaction under the studied conditions is very low, deletion of SDH3 resulted in significant changes in the expression of several genes involved in various cellular processes ranging from metabolism to the cell-cycle. By using various bioinformatics tools we explored the organization of these transcriptional changes in the metabolic and other cellular functional interaction networks. Conclusion: Our results show that the transcriptional regulatory response resulting from the impaired respiratory function is linked to several different parts of the metabolism, including fatty acid and sterol metabolism.

dna-replication

chemostat cultures

yeast

ribosomal-protein genes

stress-response

expression

metabolic network

replicating sequence binding-factor-1

cycle

complex-ii

Author

D. Cimini

Università degli Studi della Campania Luigi Vanvitelli

Technical University of Denmark (DTU)

K. R. Patil

Technical University of Denmark (DTU)

C. Schiraldi

Università degli Studi della Campania Luigi Vanvitelli

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

BMC Systems Biology

1752-0509 (ISSN)

Vol. 3 17 (artno)- 17

Subject Categories

Industrial Biotechnology

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1186/1752-0509-3-17

More information

Latest update

2/28/2018