Global transcriptional response of Saccharomyces cerevisiae to the deletion of SDH3
Artikel i vetenskaplig tidskrift, 2009

Background: Mitochondrial respiration is an important and widely conserved cellular function in eukaryotic cells. The succinate dehydrogenase complex (Sdhp) plays an important role in respiration as it connects the mitochondrial respiratory chain to the tricarboxylic acid (TCA) cycle where it catalyzes the oxidation of succinate to fumarate. Cellular response to the Sdhp dysfunction (i.e. impaired respiration) thus has important implications not only for biotechnological applications but also for understanding cellular physiology underlying metabolic diseases such as diabetes. We therefore explored the physiological and transcriptional response of Saccharomyces cerevisiae to the deletion of SDH3, that codes for an essential subunit of the Sdhp. Results: Although the Sdhp has no direct role in transcriptional regulation and the flux through the corresponding reaction under the studied conditions is very low, deletion of SDH3 resulted in significant changes in the expression of several genes involved in various cellular processes ranging from metabolism to the cell-cycle. By using various bioinformatics tools we explored the organization of these transcriptional changes in the metabolic and other cellular functional interaction networks. Conclusion: Our results show that the transcriptional regulatory response resulting from the impaired respiratory function is linked to several different parts of the metabolism, including fatty acid and sterol metabolism.

dna-replication

chemostat cultures

yeast

ribosomal-protein genes

stress-response

expression

metabolic network

replicating sequence binding-factor-1

cycle

complex-ii

Författare

D. Cimini

Università degli Studi della Campania Luigi Vanvitelli

Danmarks Tekniske Universitet (DTU)

K. R. Patil

Danmarks Tekniske Universitet (DTU)

C. Schiraldi

Università degli Studi della Campania Luigi Vanvitelli

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

BMC Systems Biology

1752-0509 (ISSN)

Vol. 3 17 (artno)- 17

Ämneskategorier

Industriell bioteknik

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1186/1752-0509-3-17

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Senast uppdaterat

2018-02-28